![]() Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed. Store lipoparticles at -80 C or proceed to downstream assays Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation MixĤ. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagentģ. Clone your gene of interest using a TOPO™ vectorĢ. With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:ġ. ![]() Lipoparticles are enriched with expressed receptors.Proteins bud off as lipoparticles and are easily collected from the culture medium.The RO retentate from SWRO plants may contain salt ranging from 6.0 to 7.0 wt., indicating higher osmotic pressures of 5059 bar 25. Use a hand roller while flattening with a hot air pistol. Leave about three inches of overlap between each layer. Arian Edalat PhD, who ran the MembranePRO business since 2011 as VP of. Correspondingly, the osmotic pressure of seawater at 25 C is between 25 and 33 bar since the NaCl concentration varies from 3.0 to 4.0 wt. Lyon JL, Fillmore JL, Klauber MR : Arsenical air pollution Lyons RT, Pitot HC : Protein degradation in normal and beige Schaffalitzky de Muckadell O. You must sew the membrane sheets together if the area that needs waterproofing is wider than the membrane sheet’s width. Cellular quality control and mammalian post-translational processing helps produce functional protein Los Angeles, California-based Water Planet has divested its MembranePRO Services business to a group of private investors.Proteins are displayed on human (or other mammalian) cell membranes.In addition, benefits of MembranePro™ technology include: Easy to use-no enzymatic or mechanical dissociation required.Scalable-easy scale-up without the need for multiple flasks. ![]() Efficient-more than 20-fold increase in membrane protein yield compared to the adherent culture system.For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.įeatures of the Expi293™ MembranePro™ Expression System include:
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